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Product Details
Synonyms
Integrin alpha V beta 8, ITGAV&ITGB8
Source
Biotinylated Mouse ITGAV&ITGB8 Heterodimer Protein (IT8-M52W7) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 988 & Gly 22 - Leu 681 (Accession # P43406-1 & Q0VBD0-1).
Predicted N-terminus: Phe 31 | Gly 22
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Biotinylated Mouse ITGAV&ITGB8 Heterodimer Protein is produced by co-expression of ITGAV and ITGB8, has a calculated MW of 114.2 kDa (ITGAV) & 77.5 kDa (ITGB8). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB8 contains no tag but a basic tail at the C-terminus.The protein migrates as 135-145 kDa (ITGAV) and 75-85 kDa (ITGB8) under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Mouse ITGAV&ITGB8 Heterodimer Protein on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Mouse ITGAV&ITGB8 Heterodimer Protein (Cat. No. IT8-M52W7) is more than 85% and the molecular weight of this protein is around 195-245 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Mouse Latent TGFB1, His Tag (Cat. No. TG1-M5245) at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse ITGAV&ITGB8 Heterodimer Protein (Cat. No. IT8-M52W7) with a linear range of 1-20 ng/mL (QC tested).
Protocol
Immobilized Human Latent TGFB1, His Tag (Cat. No. TG1-H524x) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse ITGAV&ITGB8 Heterodimer Protein (Cat. No. IT8-M52W7) with a linear range of 2-20 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
integrin alpha V/beta 8
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
3 Details
- Highest Development Stage：
Phase 2 Clinical
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