Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™)
The protein has a calculated MW of 59.0 kDa. The protein migrates as 70-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human CD19 (20-291), Fc,Avitag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human CD19 (20-291), Fc,Avitag, premium grade (Cat. No. CD9-H82F6) is more than 90% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.
Immobilized Biotinylated Human CD19 (20-291), Fc,Avitag, premium grade (Cat. No. CD9-H82F6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a with a linear range of 0.1-4 ng/mL (QC tested).
2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1 μg/mL of Biotinylated Human CD19 (20-291), Fc,Avitag, premium grade (Cat. No. CD9-H82F6) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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