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Your Position: Home > Virus > Pre-Fusion glycoprotein F0 > RSF-V82E7

Biotinylated Human respiratory syncytial virus A (strain A2) Pre-F0 Protein, His,Avitag™(MALS verified)

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.

  • Source
    Biotinylated Human respiratory syncytial virus A (strain A2) Pre-F0, His,Avitag(RSF-V82E7) is expressed from human 293 cells (HEK293). It contains AA Gln 26- Leu 513 (Accession # P03420 ).
    Predicted N-terminus: Gln 26
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

    The protein has a calculated MW of 57.8 kDa. The protein migrates as 65-67 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium Citrate,0.15 M NaCl,pH5.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Pre-Fusion glycoprotein F0 SDS-PAGE

Biotinylated Human respiratory syncytial virus A (strain A2) Pre-F0, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Pre-Fusion glycoprotein F0 MALS images

The purity of Biotinylated Human respiratory syncytial virus A (strain A2) Pre-F0, His,Avitag (Cat. No. RSF-V82E7) is more than 90% and the molecular weight of this protein is around 168-205 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Pre-Fusion glycoprotein F0 ELISA

Immobilized Anti-Fusion glycoprotein F0 Antibody, Human IgG1 (D25) at 5 μg/mL (100 μL/well) can bind Biotinylated Human respiratory syncytial virus A (strain A2) Pre-F0, His,Avitag(MALS verified) (Cat. No. RSF-V82E7) with a linear range of 0.1-8 ng/mL (QC tested).

 Pre-Fusion glycoprotein F0 ELISA

Immobilized Anti-Fusion glycoprotein F0 Antibody, Mouse IgG2a (101F) at 5 μg/mL (100 μL/well) can bind Biotinylated Human respiratory syncytial virus A (strain A2) Pre-F0, His,Avitag(MALS verified) (Cat. No. RSF-V82E7) with a linear range of 0.2-16 ng/mL (QC tested).

 Pre-Fusion glycoprotein F0 ELISA

Immobilized Recombinant Mouse Anti-RSV F Antibody (5C4) at 5 μg/mL (100 μL/well) can bind Biotinylated Human respiratory syncytial virus A (strain A2) Pre-F0, His,Avitag(MALS verified) (Cat. No. RSF-V82E7) with a linear range of 0.2-16 ng/mL (QC tested).

  • Background
    Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. The RSV fusion glycoprotein (RSV F) is the principal target of RSV neutralizing antibodies in human sera. The RSV F is a type I viral fusion protein synthesized as inactive, single-chain polypeptides that assemble into trimers. RSV F fuses the viral and host cell membranes by irreversible protein refolding from the labile prefusion conformation to the stable post-fusion conformation.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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