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Your Position: Home > Protein > Siglec-3 > CD3-H5257

Human Siglec-3 / CD33 Protein, Fc Tag (MALS verified)

  • Synonym
    CD33,SIGLEC3,gp67
  • Source
    Human Siglec-3, Fc Tag (CD3-H5257) is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 259 (Accession # AAH28152.1).
    Predicted N-terminus: Asp 18
  • Molecular Characterization
    Online(Asp 18 - His 259) AAH28152.1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 52.9 kDa. The protein migrates as 64-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Siglec-3, Fc Tag (Cat. No. CD3-H5257) SDS-PAGE gel

Human Siglec-3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human Siglec-3, Fc Tag (Cat. No. ) MALS images

The purity of Human Siglec-3, Fc Tag (Cat. No. CD3-H5257) is more than 90% and the molecular weight of this protein is around 115-145 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human Siglec-3, Fc TagHuman Siglec-3, Fc Tag (Cat. No. CD3-H5257) ELISA bioactivity

Immobilized Human Siglec-3 Antibody at 1 μg/mL (100 μL/well) can bind Human Siglec-3, Fc Tag (Cat. No. CD3-H5257) with a linear range of 0.2-2 ng/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Siglec-3 MAb (Mouse IgG1) on AMC Biosensor, can bind Human Siglec-3, Fc Tag (Cat. No. CD3-H5257) with an affinity constant of 0.329 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Myeloid cell surface antigen CD33 is also known as SIGLEC3, Siglecs (sialic acid binding Ig­like lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-­terminal Ig-­like V­-type domain, one Ig­-like C2-­type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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