This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 43.4 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human 4-1BB, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human 4-1BB, Fc Tag (Cat. No. 41B-H5258) was more than 90% and the molecular weight of this protein is around 90-110 kDa verified by SEC-MALS.
Immobilized Human 4-1BB, Fc Tag (Cat. No. 41B-H5258) at 0.1 μg/mL (100 μL/well) can bind Biotinylated Human 4-1BB Ligand, Fc,Avitag (Cat. No. 41L-H82F9) with a linear range of 0.4-6 ng/mL (QC tested).
Loaded Human 4-1BB, Fc Tag (Cat. No. 41B-H5258) on Protein A Biosensor, can bind Human 4-1BB Ligand, His Tag (Cat. No. 41L-H5249) with an affinity constant of 1.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Flow Cytometry assay shows that Human 4-1BB, Fc Tag (Cat. No. 41B-H5258) can bind to 293T cells overexpressing Human 4-1BBL. The concentration of 4-1BB used is 0.1 μg/mL (Routinely tested).
Authors: V Brucklacher-waldert, C Edwards, J Legg.
Journal: US20200362047A1 2020
Application: Binding Assay
Authors: P Bobrowicz, et al
Journal: US20190060454A1 2019
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Price(USD) : $290.00
Price(USD) : $1950.00
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