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This protein carries no "tag"
The protein has a calculated MW of 13.6 kDa. The protein migrates as 16-17 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human Midkine Protein, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human Midkine Protein, premium grade (Cat. No. MIE-H5117) was greater than 95% as determined by SEC-HPLC.
Immobilized Human Midkine Protein, premium grade (Cat. No. MIE-H5117) at 2 μg/mL (100 μL/well) can bind Human Glypican 2, His Tag (Cat. No. GP2-H52H3) with a linear range of 1-63 ng/mL (QC tested).
Immobilized Human Midkine Protein, premium grade (Cat. No. MIE-H5117) at 2 μg/mL (100 μL/well) can bind Human LRP-6 (20-630), Mouse IgG2a Fc Tag (Cat. No. LR6-H5253) with a linear range of 1-31 ng/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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