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Your Position: Home > TME Relevant Pathway > MAdCAM-1 > MAM-H8251

Biotinylated Human MAdCAM-1 Protein, Fc,Avitag™ (MALS verified)

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.

  • Synonym
    MAdCAM-1,Mucosal vascular addressin cell adhesion molecule 1 transcript variant 1
  • Source
    Biotinylated Human MAdCAM-1, Fc Tag(MAM-H8251) is expressed from human 293 cells (HEK293). It contains AA Gln 19- Gln 333 (Accession # Q4PKD0-1 ).
    Predicted N-terminus: Gln 19
  • Molecular Characterization
    MAdCAM-1 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™)

    The protein has a calculated MW of 63 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
MAdCAM-1 SDS-PAGE

Biotinylated Human MAdCAM-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
MAdCAM-1 MALS images

The purity of Biotinylated Human MAdCAM-1, Fc Tag (Cat. No. MAM-H8251) is more than 95% and the molecular weight of this protein is around 155-175kDa verified by SEC-MALS.

Bioactivity-ELISA
 MAdCAM-1 ELISA

Immobilized Human ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT7-H52W4) at 5 μg/mL (100 μL/well) can bind Biotinylated Human MAdCAM-1 Protein, Fc,Avitag (Cat. No. MAM-H8251) with a linear range of 0.2-16 ng/mL (QC tested).

  • Background
    The mucosal addressin cell adhesion molecule-1 (MAdCAM-1) takes a key role in the endothelial adhesion and migration of lymphocytes to sites of inflammation in inflammatory disease. In vitro and in vivo data indicate that blockade of the adhesion molecule mucosal address in cell adhesion molecule (MAdCAM) pathway decreases leukocyte homing to the gut by inhibiting the interaction between MAdCAM and its ligand the α4β7 integrin expressed on lymphocytes.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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