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This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 47.2 kDa. The protein migrates as 63-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human Nogo Receptor, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Human Nogo Receptor, His Tag (Cat. No. NOR-H52H3) capture on NTA-Series S sensor chip can bind Human MAG, Fc Tag (Cat. No. MAG-H5254) with an affinity constant of 2.21 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Human MAG, Fc Tag (Cat. No. MAG-H5254) captured on Protein A Chip can bind Human Nogo Receptor, His Tag (Cat. No. NOR-H52H3) with an affinity constant of 4.81 μM as determined in SPR assay (Biacore 8K) (Routinely tested).
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