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Your Position: Home > CKP > BTLA > BTA-H82E5

Biotinylated Human BTLA (31-150) (M105V, G138S, V148M) Protein, His,Avitag™ (MALS verified)

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    BTLA,CD272
  • Source
    Biotinylated Human BTLA (31-150) (M105V, G138S, V148M), His,Avitag(BTA-H82E5) is expressed from human 293 cells (HEK293). It contains AA Lys 31 - Ser 150 (Accession # AAP44003.1 (M105V, G138S, V148M)).
    Predicted N-terminus: Lys 31
  • Molecular Characterization
    BTLA Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

    The protein has a calculated MW of 17.3 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
BTLA SDS-PAGE

Biotinylated Human BTLA (31-150) (M105V, G138S, V148M), His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
BTLA MALS images

The purity of Biotinylated Human BTLA (31-150) (M105V, G138S, V148M), His,Avitag (Cat. No. BTA-H82E5) is more than 85% and the molecular weight of this protein is around 28-36 kDa verified by SEC-MALS.

Bioactivity-ELISA
 BTLA ELISA

Immobilized Biotinylated Human BTLA (31-150) (M105V, G138S, V148M), His,Avitag (Cat. No. BTA-H82E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human HVEM, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. HVM-H5255) with a linear range of 1-20 ng/mL (QC tested).

 BTLA ELISA

Immobilized Biotinylated Human BTLA (31-150) (M105V, G138S, V148M), His,Avitag (Cat. No. BTA-H82E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human HVEM, Fc Tag (Cat. No. HVM-H5258) with a linear range of 0.2-10 ng/mL (Routinely tested).

  • Background
    B- and T-lymphocyte attenuator (BTLA) is also known as B- and T-lymphocyte-associated protein, CD antigen CD272. BTLA contains one Ig-like V-type (immunoglobulin-like) domain. As a lymphocyte inhibitory receptor, BTLA / CD272 inhibits lymphocytes during immune response. BTLA / CD272 can interact with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2, and interact with TNFRSF14/HVEM.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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