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Your Position: Home > Immune Checkpoint Proteins > SIRP alpha > Human SIRP alpha /CD172A Protein, With C-mFc Tag

Human SIRP alpha / CD172a Protein, mouse IgG1 Fc Tag (HPLC-verified)

  • Synonym
    SHPS1,SIRPA,CD172A,BIT,MFR,MYD1,P84,PTPNS1
  • Source
    Human SIRP alpha, mouse IgG1 Fc Tag(HPLC-verified) (SIA-H52A8) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Arg 370 (Accession # NP_001035111).
    Predicted N-terminus: Glu 31
  • Molecular Characterization
    SIRP alpha(Glu 31 - Arg 370) NP_001035111

    This protein carries a mouse IgG1 Fc tag at the C-terminus, and has a calculated MW of 63.5 kDa. The predicted N-terminus is Glu 31. The reducing (R) protein migrates as 70-105 kDa in SDS-PAGE due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human SIRP alpha, mouse IgG1 Fc Tag(HPLC-verified) (Cat. No. SIA-H52A8) SDS-PAGE gel

Human SIRP alpha, mouse IgG1 Fc Tag(HPLC-verified) on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC
Human SIRP alpha, mouse IgG1 Fc Tag(HPLC-verified) (Cat. No. ) HPLC images

The purity of Human SIRP alpha, mouse IgG1 Fc Tag (HPLC-verified) (Cat. No. SIA-H52A8) was greater than 90% as determined by SEC-HPLC.

Bioactivity
Human SIRP alpha, mouse IgG1 Fc Tag(HPLC-verified)Human SIRP alpha, mouse IgG1 Fc Tag(HPLC-verified) (Cat. No. SIA-H52A8) ELISA bioactivity

Immobilized Human CD47, His Tag (Cat. No. CD7-H5227) at 2 μg/mL can bind  Human SIRP alpha, mouse IgG1 Fc Tag (HPLC-verified) (Cat. No. SIA-H52A8) with a linear range of 8-250 ng/mL (QC tested).

FACS assay shows that recombinant Biotinylated FACS

FACS assay shows that recombinant Human SIRP alpha, mouse IgG1 Fc Tag (HPLC-verified) (Cat. No. SIA-H52A8) can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 0.3 ug/ml (Routinely tested).

FACS assay shows that recombinant Biotinylated FACS

FACS analysis shows that the binding of Human SIRP alpha, mouse IgG1 Fc Tag (HPLC-verified) (Cat. No. SIA-H52A8) to Jurkat expressing CD47 was inhibited by  increasing concentration of neutralizing anti-CD47 antibody. The concentration of SIRP alpha used is 0.3 ug/ml. IC50=0.1318 ug/ml (Routinely tested).

  • Background
    Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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