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Your Position: Home > Protein > GAD1 > GA1-H5543

Human GAD1 / GAD67 Protein, His Tag

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  • Synonym
    GAD1,GAD67,Glutamate decarboxylase 1
  • Source
    Human GAD1, His Tag(GA1-H5543) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Leu 594 (Accession # Q99259-1 ).
    Predicted N-terminus: Met 1
  • Molecular Characterization
    GAD1 Structure

    This protein carries a polyhistidine tag at the N-terminus

    The protein has a calculated MW of 68.8 kDa. The protein migrates as 66 kDa, 90 kDa and >116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >85% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM Tris, 500 mM Nacl, pH8.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
SDS-PAGE
GAD1 SDS-PAGE

Human GAD1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 85%.

Bioactivity-ELISA
 GAD1 ELISA

Immobilized Human GAD1, His Tag (Cat. No. GA1-H5543) at 1 μg/mL (100 μL/well) can bind Anti-GAD1 Antibody with a linear range of 1-8 ng/mL (QC tested).

  • Background
    Glutamate decarboxylase or glutamic acid decarboxylase (GAD) is an enzyme that catalyzes the decarboxylation of glutamate to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. GAD uses pyridoxal-phosphate (PLP) as a cofactor.Two molecular forms of GAD (65 kDa and 67 kDa) are highly conserved and both are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. The isoforms are regionally distributed cytoplasmically in the brains of rats and mice. GAD65 is an ampiphilic, membrane-anchored protein (585 a.a.), and is responsible for vesicular GABA production. GAD67 is cytoplasmic (594 a.a.), and seems to be responsible for significant cytoplasmic GABA production.
  • Clinical and Translational Updates

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ETD of in-stock products: 2 business days

Price(USD) : $350.00

Price(USD) : $2310.00

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