Rat ITGAV&ITGB6 Heterodimer, His Tag&Tag Free, has a calculated MW of 112.3 kDa (ITGAV) & 80.1 kDa (ITGB6). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB6 contains no tag but a basic tail at the C-terminus.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM NaCl,pH7.5 with trehalose as protectant.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Rat ITGAV&ITGB6 Heterodimer, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Rat ITGAV&ITGB6 Heterodimer, His Tag&Tag Free (Cat. No. IT6-R52E6) is more than 85% and the molecular weight of this protein is around 242-272 kDa verified by SEC-MALS.
Immobilized Human Fibronectin at 5 μg/mL (100 μL/well) can bind Rat ITGAV&ITGB6 Heterodimer, His Tag&Tag Free (Cat. No. IT6-R52E6) with a linear range of 0.02-0.625 μg/mL (QC tested).
Immobilized Rat ITGAV&ITGB6 Heterodimer, His Tag&Tag Free (Cat. No. IT6-R52E6) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Latent TGF-Beta 1, His,Avitag (Cat. No. TG1-H82Qb) with a linear range of 0.3-10 ng/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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