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Your Position: Home > Protein > IL-12 R beta 1 > ILB-C52E2

Biotinylated Cynomolgus IL-12 R beta 1 / CD212 Protein, His,Avitag™

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  • Synonym
    IL-12 R beta 1,IL12RB1,CD212,IL12R,IL12RB,IL-12RB1
  • Source
    Biotinylated Cynomolgus IL-12 R beta 1 , His,Avitag(ILB-C52E2) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Asp 545 (Accession # G7PWY9-1).
    Predicted N-terminus: Cys 24
  • Molecular Characterization
    IL-12 R beta 1 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

    The protein has a calculated MW of 61.0 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-12 R beta 1 SDS-PAGE

Biotinylated Cynomolgus IL-12 R beta 1 , His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 IL-12 R beta 1 ELISA

Immobilized Biotinylated Cynomolgus IL-12 R beta 1 , His,Avitag (Cat. No. ILB-C52E2) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human IL-12B, Fc Tag (Cat. No. NK2-H5258) with a linear range of 0.1-8 ng/mL (QC tested).

Bioactivity-BLI
 IL-12 R beta 1 BLI

Loaded Biotinylated Cynomolgus IL-12 R beta 1 , His,Avitag (Cat. No. ILB-C52E2) on SA Biosensor, can bind ActiveMax® Human IL-12 ,Tag Free (Cat. No. IL2-H5210) with an affinity constant of 1.9 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    The human IL-12 R subunit is a member of the cytokine receptor superfamily and the functional high-affinity IL-12R is composed of at least two beta-type cytokine receptor subunits, each independently exhibiting a low affinity for IL-12. IL-12 R beta 1 (Interleukin-12 receptor subunit beta-1) is also known as IL-12RB1, CD212. Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.
  • Clinical and Translational Updates

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  • Latest Research Phase:NDA/BLA

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