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Your Position: Home > > MMP-3 > MM3-H52H3

Human MMP-3 Protein, His Tag (active enzyme, MALS verified)

  • Synonym
    CHDS6
  • Source
    Human MMP-3, His Tag(MM3-H52H3) is expressed from human 293 cells (HEK293). It contains AA Tyr 18 - Thr 272 (Accession # P08254-1 ).
    Predicted N-terminus: Tyr 18
  • Molecular Characterization
    MMP-3 Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 30.7 kDa. The protein migrates as 32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 25 mM MES,150 mM NaCl,pH 6.0 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -20°C or lower upon receipt;
    2. -20°C for 3 months under sterile conditions.
SDS-PAGE
MMP-3 SDS-PAGE

Human MMP-3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
MMP-3 MALS images

The purity of Human MMP-3, His Tag (Cat. No. MM3-H52H3) is more than 85% and the molecular weight of this protein is around 30-43kDa verified by SEC-MALS.

Bioactivity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 . The specific activity is >422 pmol/min/µg (QC tested).

  • Background
    Matrix metalloproteinase 3 (MMP-3), also known as stromelysin 1 and progelatinase, is encoded by the MMP3 gene. The expression of MMP3 is mainly regulated at the transcriptional level, where gene promoters respond to a variety of stimuli including growth factors, cytokines, carcinogens and proto-oncogene products. MMP-3 degrade II, III, IV, IX and X collagen, proteoglycan, fibronectin, laminin, and elastin. In addition, MMP-3 can also activate other MMPs such as MMP-1, MMP-7 and MMP-9, so MMP-3 plays a key role in connective tissue remodeling. This enzyme is involved in wound repair, atherosclerotic progression, and tumor initiation. Studies of MMP-3 wild type and gene knockout in mice have shown that MMP-3 increases the permeability of the blood-brain barrier after traumatic injury. Nuclear MMP-3 works as a transcription factor and protease.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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ETD of in-stock products: 2 business days

Price(USD) : $350.00

Price(USD) : $2160.00

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Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

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