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Your Position: Home > CKP > EphA10 > EP0-H52H3

Human EphA10 Protein, His Tag

  • Synonym
    Ephrin type-A receptor 10
  • Source
    Human EphA10, His Tag(EP0-H52H3) is expressed from human 293 cells (HEK293). It contains AA Pro 27 - Pro 564 (Accession # Q5JZY3-1 ).
    Predicted N-terminus: Pro 27
  • Molecular Characterization
    EphA10 Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 60.2 kDa. The protein migrates as 65-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
EphA10 SDS-PAGE

Human EphA10, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
 EphA10 ELISA

Immobilized Human EphA10, His Tag (Cat. No. EP0-H52H3) at 5 μg/mL (100 μL/well) can bind Human Ephrin-A3, Fc Tag (Cat. No. EA3-H5258) with a linear range of 0.1-2 ng/mL (QC tested).

 EphA10 ELISA

Immobilized Human Ephrin-A3, Fc Tag (Cat. No. EA3-H5258) at 5 μg/mL (100 μL/well) can bind Human EphA10, His Tag (Cat. No. EP0-H52H3) with a linear range of 0.005-0.313 μg/mL (Routinely tested).

  • Background
    At present, the proportion of breast cancer, cervical cancer and ovarian cancer in the occurrence of tumors that cause womens death has remained high. EPHA10 belongs to the EPH family, which is the largest known tyrosine kinase family, with 16 members. These members play a normal physiological function after interacting with their ligand Ephrin, and have been studied in sugar metabolism, new tissue construction, angiogenesis and other aspects. EPHA10 gene is located in 1p34 and mainly expressed in testis.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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