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Product Details
Synonyms
Ephrin type-A receptor 10
Source
Human EphA10, His Tag (EP0-H52H3) is expressed from human 293 cells (HEK293). It contains AA Pro 27 - Pro 564 (Accession # Q5JZY3-1).
Predicted N-terminus: Pro 27
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 60.2 kDa. The protein migrates as 65-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human EphA10, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-ELISA
Immobilized Human EphA10, His Tag (Cat. No. EP0-H52H3) at 5 μg/mL (100 μL/well) can bind Human Ephrin-A3, Fc Tag (Cat. No. EA3-H5258) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized Human Ephrin-A3, Fc Tag (Cat. No. EA3-H5258) at 5 μg/mL (100 μL/well) can bind Human EphA10, His Tag (Cat. No. EP0-H52H3) with a linear range of 0.005-0.313 μg/mL (Routinely tested).
Protocol
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