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Your Position: Home > Cytokines > IL-10 R alpha > ILR-H525a

Human IL-10 R alpha / CD210 Protein, Fc Tag (MALS verified)

  • Synonym
    Interleukin-10 receptor subunit alpha,IL-10 receptor subunit alpha,IL-10R subunit alpha,IL-10RA,CDw210a,Interleukin-10 receptor subunit 1,IL-10R subunit 1,IL-10R1,CD210,IL10RA,IL10R
  • Source
    Human IL-10 R alpha / CD210, Fc Tag (ILR-H525a) is expressed from human 293 cells (HEK293). It contains AA His 22 - Asn 235 (Accession # Q13651 ).
    Predicted N-terminus: His 22
  • Molecular Characterization
    IL-10 R alpha Structure

    This protein carries a human IgG1 Fc tag at the C-terminus

    The protein has a calculated MW of 50.8 kDa. The protein migrates as 65-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-10 R alpha SDS-PAGE

Human IL-10 R alpha / CD210, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
IL-10 R alpha MALS images

The purity of Human IL-10 R alpha / CD210, Fc Tag (Cat. No. ILR-H525a) is more than 85% and the molecular weight of this protein is around 125-150kDa verified by SEC-MALS.

Bioactivity-ELISA
 IL-10 R alpha ELISA

Immobilized Human IL-10, His Tag (Cat. No. IL0-H4248) at 5 μg/mL (100 μL/well) can bind Human IL-10 R alpha / CD210, Fc Tag (Cat. No. ILR-H525a) with a linear range of 0.2-2 ng/mL (QC tested).

Bioactivity-BLI
 IL-10 R alpha BLI

Loaded Human IL-10 R alpha / CD210, Fc Tag (Cat. No. ILR-H525a) on Protein A Biosensor, can bind Human IL-10, His Tag (Cat. No. IL0-H4248) with an affinity constant of 1.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Interleukin-10 receptor subunit alpha (IL-10 R alpha ) is a cell surface receptor for the cytokine IL-10 that participates in IL-10 mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well.In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively.These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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