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Your Position: Home > Cell > Fc gamma RIIB / CD32b > SCCHO-ATP060M

CHO/Human CD32b Stable Cell Line (Medium Expression) Development Service

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  • Description
    The CHO/Human CD32b Stable Cell Line was engineered to express full length human CD32b receptor (Gene ID: 2213), with different levels of CD32b expression (High, Medium, Low), which can be used to test agonist antibody whether in a CD32b-dependent manner to strengthen the agonistic activity. When co-cultured with Human 4-1BB HEK293 Reporter Cell and anti-4-1BB agonist antibody, the anti-4-1BB antibody can be crosslinked, thereby strengthening 4-1BB pathway-activated luminescence.
  • Application

    • Useful for cell-based CD32b binding assay

    • Useful for CD32b-mediated crosslinking

     Fc gamma RIIB / CD32b Assay Principles

  • Growth Properties
    Adherent
  • Selection Marker
    Hygromycin (20 μg/mL)
  • Culture Medium
    F-12K+ 10% FBS
  • Freeze Medium
    Serum-free cell cryopreservation medium
  • Quantity
    1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
  • Storage
    Frozen in liquid nitrogen.
  • Mycoplasma Testing
    Negative
  • Sterility Testing
    Negative
  • Instructions for Use
    See data sheet for detailed culturing and assay protocol.
Receptor Assay
 Fc gamma RIIB / CD32b FACS

Expression analysis of human CD32b on CHO/Human CD32b Stable Cell Line by FACS.
Cell surface staining using PE/Cy7-labeled anti-human CD32b antibody was performed on CHO/Human CD32b Stable Cell Line with different expression levels: CHO/Human CD32b Stable Cell Line (Low Expression); CHO/Human CD32b Stable Cell Line (Medium Expression); CHO/Human CD32b Stable Cell Line (High Expression).

Application
 Fc gamma RIIB / CD32b APPLICATION

Bioactivity analysis of anti-human 4-1BB antibody through CHO/Human CD32b Stable Cell Line (Medium Expression) crosslinking to test whether in a CD32b-dependent manner to strengthen the agonistic activity.
The EC50 of anti-human 4-1BB antibody is approximately 0.18 μg/mL through CHO/Human CD32b Stable Cell Line (Medium Expression) crosslinking.

Please contact us if you are interested in related cell pool service.

  • Background
    Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. Low affinity immunoglobulin gamma Fc region receptor II-b (FCGR2B) is also known as CD32b, FCG2, IGFR2. CD32B is expressed on B cells and myeloid dendritic cells. Ligation of CD32B on B cells downregulates antibody production and may, in some circumstances, promote apoptosis. Co-ligation of CD32B on dendritic cells inhibits maturation and blocks cell activation. CD32B may also be a target for monoclonal antibody therapy for malignancies.
  • Permits & Restrictions
    This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
  • Clinical and Translational Updates

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ETA of in-stock products: 2 business days

Price(USD) : $3500.00

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:5 Details
  • Latest Research Phase:Phase 3 Clinical

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