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Your Position: Home > Cell > Fc gamma RI / CD64 > SCCHO-ATP062M

CHO/Human CD64 Stable Cell Line (Medium Expression) Development Service

  • Description
    The CHO/Human CD64 Stable Cell Line was engineered to express full length human CD64 receptor (Gene ID: 2209), with different levels of CD64 expression (High, Medium, Low), which can be used to test agonist antibody whether in a CD64-dependent manner to strengthen the agonistic activity. When co-cultured with Human 4-1BB (Luc) HEK293 Reporter Cell (Cat.No.CHEK-ATF073) and anti-4-1BB agonist antibody, the anti-4-1BB antibody can be crosslinked, thereby strengthening 4-1BB pathway-activated luminescence.
  • Application

    • Useful for cell-based CD64 binding assay

    • Useful for CD64-mediated crosslinking in reporter gene assay

     Fc gamma RI / CD64 Assay Principles

  • Growth Properties
  • Selection Marker
    Hygromycin (20 μg/mL)
  • Culture Medium
    F-12K+ 10% FBS
  • Freeze Medium
    Serum-free cell cryopreservation medium
  • Quantity
    1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
  • Storage
    Frozen in liquid nitrogen.
  • Instructions for Use
    See data sheet for detailed culturing and assay protocol.
Receptor Assay
 Fc gamma RI / CD64 FACS

Expression analysis of human CD64 on CHO/Human CD64 Stable Cell Line by FACS.
Cell surface staining using PE-labeled anti-human CD64 antibody was performed on CHO/Human CD64 Stable Cell Line with different expression levels: CHO/Human CD64 Stable Cell Line (Low Expression); CHO/Human CD64 Stable Cell Line (Medium Expression); CHO/Human CD64 Stable Cell Line (High Expression).


Bioactivity analysis of anti-human 4-1BB antibody through CHO/Human CD64 Stable Cell Line (Medium Expression) crosslinking to test whether in a CD64-dependent manner to strengthen the agonistic activity.
The EC50 of anti-human 4-1BB antibody is approximately 0.49 μg/mL through CHO/Human CD64 Stable Cell Line (Medium Expression) crosslinking.

  • Background
    Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. High affinity immunoglobulin gamma Fc receptor I is also known as FCGR1A, FCG1, FCGR1, CD64 and IGFR1, is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity, which belongs to the immunoglobulin superfamily or FCGR1 family. FCGR1A / CD64 contains 3 Ig-like C2-type (immunoglobulin-like) domains. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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ETD of in-stock products: 2 business days

Price(USD) : $3150.00

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:0 Details
  • Latest Research Phase:Discontinued

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