Cynomolgus ALPP Protein, His Tag (active enzyme)

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Cat. No. / Size
Price
Qty
ALP-C52H3-100ug
$390.00
ALP-C52H3-1mg
$2680.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    PLAP

  • Source

    Cynomolgus ALPP, His Tag (ALP-C52H3) is expressed from human 293 cells (HEK293). It contains AA Ile 21 - Asp 504 (Accession # XP_045223825.1).

    Predicted N-terminus: Ile 21

    Request for sequence
  • Molecular Characterization

    ALPP Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 54.6 kDa. The protein migrates as 65-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    ALPP SDS-PAGE

    Cynomolgus ALPP, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     ALPP ELISA

    Immobilized Cynomolgus ALPP, His Tag (Cat. No. ALP-C52H3) at 1 μg/mL (100 μL/well) can bind various dilution ratio of Anti-ALPP/ALPG/ALPI Polyclonal Antibody (QC tested).

    Protocol
  • Bioactivity

    Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is >450 pmol/min/μg (QC tested).

    Protocol

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Background

In most mammals there are four different isozymes: placental (ALPP), germ cell (ALPG), intestinal (ALPI) and tissue non-specific (liver/bone/kidney) (ALPL/TNAP). ALPP is also known as Alkaline phosphatase, placental type and PLAP. Alkaline phosphatase that can hydrolyze various phosphate compounds.

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Drug Development Progress
  • English Name:

    Alkaline phosphatase, placental type

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    1 Details

  • Highest Development Stage:

    Phase 2 Clinical

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