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Product Details
Synonyms
GLP-1-R, GLP-1R, glucagon like peptide 1 receptor
Source
Biotinylated Human GLP1R, His,Avitag (GLR-H82E3) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Glu 139 (Accession # P43220-1).
Predicted N-terminus: Ala 21
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 17.3 kDa. The protein migrates as 30-34 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human GLP1R, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Biotinylated Human GLP1R, His,Avitag (Cat. No. GLR-H82E3) was greater than 85% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized Biotinylated Human GLP1R Protein, His,Avitag (Cat. No. GLR-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-GLP-R1 Antibody with a linear range of 0.5-31 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Anti-GLPR1 antibody captured on Protein A Chip can bind Biotinylated Human GLP1R, His,Avitag (Cat. No. GLR-H82E3) with an affinity constant of 13.1 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Biotinylated Human GLP1R, His,Avitag (Cat. No. GLR-H82E3) immobilized on CM5 Chip can bind GLP-1 (7-37) with an affinity constant of 1.33 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human GLP1R, His,Avitag (Cat. No. GLR-H82E3) on NTA Biosensor, can bind GLP-1 (7-37) with an affinity constant of 1.40 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Glucagon-like peptide-1
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
13 Details
- Highest Development Stage：
Phase 3 Clinical
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