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Your Position: Home > Kits > IL-6 > CRS-B001

ClinMax™ Human IL-6 ELISA Kit

For research use only.

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Product Details
Assay TypeSandwich-ELISA
Regulatory StatusRUO
Sensitivity<2.0 pg/mL
Standard Curve Range6.25 pg/mL-400 pg/mL
Assay Time2 hr
Suitable Sample TypeFor the quantitative determination of human IL-6 in serum, plasma and cell supernatant.
Sample volume50 μL
Materials Provided
CRB001-C01Pre-coated Anti-IL-6 Antibody Microplate1 plate
CRB001-C02Human IL-6 Standard20 μg×2
CRB001-C03Biotin-Anti-IL-6 Antibody Con. Solution100 μL
CRB001-C04Biotin-Antibody Dilution Buffer8 mL
CRB001-C05Streptavidin-HRP Con. Solution500 μL
CRB001-C06Streptavidin-HRP Dilution Buffer15 mL
CRB001-C0720× Washing Buffer50 mL
CRB001-C081× Dilution Buffer15 mL×2
CRB001-C09Substrate Solution12 mL
CRB001-C10Stop Solution6 mL
  • Background
    ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human IL-6 that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.

    A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.

    ClinMax™ ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.

  • Application

    The kit is developed for quantitative detection of IL-6 in human serum and cell culture supernates.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit is used to measure the concentrations of human Interleukin 6 (IL-6) by employing a standard sandwich-ELISA format. The microplate in the kit has been pre-coated with Anti- IL-6 Antibody. First add the standard samples provided in kit and your samples to the plate, next add detection antibody Biotin-Anti-IL-6 Antibody to the plate, incubate and wash the wells. After wash add HRP-Streptavidin to the plate, incubate and wash the wells. Lastly load the substrate into the wells and color develops in proportion to the amount of Interferon-γ bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm and 630nm.
Typical Data Please refer to DS document for The assay protocol.

For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of CRS-B001 is less than 2.0 pg/mL.

  • Background: IL-6
    Interleukin 6, IL-6
    Interleukin-6 (IL-6) is a member of the pro-inflammatory cytokine family. It induces the expression of a variety of proteins responsible for acute inflammation, and plays an important role in the proliferation and differentiation of cells in humans. It has been demonstrated the dysregulation of the IL-6 signaling pathways can result in inflammatory and autoimmune disorders as well as cancer development.
    Human IL-6 (also known as B-cell stimulatory factor 2, Interferon beta-2, Hybridoma growth factor, and CTL differentiation factor) plays an essential role in the final differentiation of B-cells into Ig-secreting cells. It induces myeloma and plasmacytoma growth, nerve cells differentiation, and acute phase reactants in hepatocytes. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, and keratinocytes. It has been shown that IL-6 plays a critical role in many physiological and pathological conditions, including autoimmune diseases and rheumatoid arthritis.
  • Clinical and Translational Updates
  • Please contact us via if you have any question on this product.

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