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Your Position: Home > Protein > Integrin alpha 6 beta 4 > IN4-H52W3

Human Integrin alpha 6 beta 4 Protein, His Tag&Tag Free (MALS verified)

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  • Synonym
    Integrin alpha 6 beta 4, ITGA6&ITGB4
  • Source
    Human Integrin alpha 6 beta 4 Protein, His Tag&Tag Free(IN4-H52W3) is expressed from human 293 cells (HEK293). It contains AA Phe 24 - Gly 1012 | Asn 28 - Ser 710 (Accession # P23229-2 & P16144-2 ).
    Predicted N-terminus: Phe 24 | Asn 28
  • Molecular Characterization
    Integrin alpha 6 beta 4 Structure

    Human Integrin alpha 6 beta 4 Protein, His Tag&Tag Free, produced by co-expression of ITGA6 and ITGB4, has a calculated MW of 116.8 kDa (ITGA6) and 81.8 kDa (ITGB4). Subunit ITGA6 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB4 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 130-140 kDa (ITGA6) and 75-85 kDa (ITGB4) respectively due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM NaCl,0.2 M Arginine,pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Integrin alpha 6 beta 4 SDS-PAGE

Human Integrin alpha 6 beta 4 Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Integrin alpha 6 beta 4 MALS images

The purity of Human Integrin alpha 6 beta 4 Protein, His Tag&Tag Free (Cat. No. IN4-H52W3) is more than 90% and the molecular weight of this protein is around 195-220 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Integrin alpha 6 beta 4 ELISA

Immobilized Human Integrin alpha 6 beta 4 Protein, His Tag&Tag Free (Cat. No. IN4-H52W3) at 5 μg/mL (100 μL/well) can bind Human Laminin 511 Protein, premium grade (Cat. No. LA8-H5283) with a linear range of 0.8-13 ng/mL (QC tested).

 Integrin alpha 6 beta 4 ELISA

Immobilized Human Laminin 511 Protein, premium grade (Cat. No. LA8-H5283) at 5 μg/mL (100 μL/well) can bind Human Integrin alpha 6 beta 4 Protein, His Tag&Tag Free (Cat. No. IN4-H52W3) with a linear range of 0.01-0.156 μg/mL (Routinely tested).

  • Background
    Human integrin alpha(10)I domain as a recombinant protein to reveal its ligand binding specificity. In general, alpha(10)I did recognize collagen types I-VI and laminin-1 in a Mg(2+)-dependent manner, whereas its binding to tenascin was only slightly better than to albumin. Alpha 10 beta 1 is a known collagen-binding I domain integrin, in addition to α1β1, α2β1 and α11β1. GROGER found in the N-terminal domain of collagens I and III, is only weakly recognised by α10β1, an important collagen receptor on chondrocytes, contrasting with the other collagen-binding integrins.
  • Clinical and Translational Updates

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