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Product Details
Source
Anti-SARS-CoV-2 Spike RBD Antibody, Human IgG1 (10B1A5) (BA.2&BA.4&BA.5/Omicron Specific) is isolated from a Spike RBD infected mouse and is recombinantly produced in human 293 cells (HEK293).
Antibody Type
Recombinant Monoclonal
Clone
10B1A5
Isotype
Human IgG1, Kappa
Host Species
Mouse
Immunogen
Recombinant SARS-CoV-2 Spike Trimer Protein (BA.4/Omicron) erived from human 293 cells
Specificity
This product is a specific antibody specifically reacts with Spike RBD.
Purification
Protein A purified / Protein G purified.
Aggregation
Less than 5%, as determined by SEC-MALS.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
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Performance Data
Bioactivity-ELISA
Immobilized SARS-CoV-2 Spike RBD, His Tag (BA.2/Omicron) (Cat. No. SPD-C522g) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Human IgG1 (10B1A5) (BA.2&BA.4&BA.5/Omicron Specific) (Cat. No. SPD-M541) with a linear range of 0.5-63 ng/mL (QC tested).
Protocol
Immobilized SARS-CoV-2 Spike RBD, His Tag (BA.2.12.1/Omicron) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Human IgG1 (10B1A5) (BA.2&BA.4&BA.5/Omicron Specific) (Cat. No. SPD-M541) with a linear range of 0.2-16 ng/mL (QC tested).
Protocol
Immobilized SARS-CoV-2 Spike RBD, His Tag (BA.4&BA.5/Omicron) (Cat. No. SPD-C522r) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Human IgG1 (10B1A5) (BA.2&BA.4&BA.5/Omicron Specific) (Cat. No. SPD-M541) with a linear range of 0.5-16 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Anti-SARS-CoV-2 Spike RBD Antibody, Human IgG1 (10B1A5) (BA.2&BA.4&BA.5/Omicron Specific) (Cat. No. SPD-M541) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind SARS-CoV-2 Spike RBD, His Tag (BA.4&BA.5/Omicron) (Cat. No. SPD-C522r) with an affinity constant of 15.5 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Anti-SARS-CoV-2 Spike RBD Antibody, Human IgG1 (10B1A5) (BA.2&BA.4&BA.5/Omicron Specific) (Cat. No. SPD-M541) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind SARS-CoV-2 Spike RBD, His Tag (BA.2.12.1/Omicron) with an affinity constant of 11.9 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Anti-SARS-CoV-2 Spike RBD Antibody, Human IgG1 (10B1A5) (BA.2&BA.4&BA.5/Omicron Specific) (Cat. No. SPD-M541) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind SARS-CoV-2 Spike RBD, His Tag (BA.2/Omicron) (Cat. No. SPD-C522g) with an affinity constant of 11.1 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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