Acrobiosystems for English
icon_bulk_orderBulk inquiry/Quick order
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Antibody > Mouse IgG > AMG-S163

Anti-Mouse IgG-Acridinium ester (MALS verified)

Order Now

  • Source
    Anti-Mouse IgG-Acridinium ester is a chimeric monoclonalantibody recombinantly expressed from human 293 cells(HEK293), which combines the variable region of a mousemonoclonal antibody with rabbit IgG constant domain.
  • Isotype
    Rabbit IgG | Kappa
  • Specificity
    This product is a specific antibody specifically reacts with Mouse IgG.
  • Labeling
    Acridinium ester, can react with the primary amino group of protein. Under alkaline conditions, NHS is replaced as the leaving group, and the protein forms a stable amide bond with Acridinium ester.
  • Protein Ratio
    Passed as determined by binding MPCLIA.
  • Application
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH6.3 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

Anti-Mouse IgG-Acridinium ester on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Mouse IgG MALS images

The purity of Anti-Mouse IgG-Acridinium ester (Cat. No. AMG-S163) is more than 90% and the molecular weight of this protein is around 135-155 kDa verified by SEC-MALS.


Immobilized 0.04 μg /Test of Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E4) to the Streptavidin-Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A006, 20 μg beads/Test), incubated with 100 μL /Test of Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (Cat. No. PD1-H52A3) at increasing concentration coupled to Anti-Mouse IgG-Acridinium ester (Cat. No. AMG-S163, 0.04 μg /Test). Detection was performed with sensitivity of 1.5625 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).


The MPCLIA assay shows that Anti-Mouse IgG-Acridinium ester (Cat. No. AMG-S163) is stable at 37°C for 48 hours.


The MPCLIA assay shows that Anti-Mouse IgG-Acridinium ester (Cat. No. AMG-S163) is stable after freezing and thawing 3 times.

  • Background
    Chemiluminescence immunoassay (CLIA) is a much more effective measurement with the advantages of high sensitivity, uniformity and broad dynamic range.
    However, in traditional chemiluminescent immunoassay (CLIA),microplate was applied to immobilize antigen or antibody by physical absorption, which holds a negative effect on the perfor-mance of assay because of the low specific surface area of micro-plate.
    The use of micromagnetic particles (MMPs) have proven to be a support for addressing the shortage of traditional CLIA mentioned above The chemiluminescent immunoassay is a technique used for disease diagnosis, drug development, chemical reaction monitoring, and many other applications.
    The MMPs-based chemiluminescence immunoassays could improve the surface area for immobilization, capture efficiency and accuracy of the assay, thus obtaining higher sensitivity and shorter analysis time.
  • Clinical and Translational Updates

Comments (0)

ETA of in-stock products: 2 business days

Price(USD) : $54.00

Price(USD) : $522.00

Promotion & Exhibitions

Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Related Products

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

Call us
Call us
North America:
+1 800-810-0816 (Toll Free)
Asia & Pacific:
+86 400-682-2521
+1 888-377-6111
1 Innovation Way, Newark, DE 19711, USA

Leave a message