Product Details
Synonyms
LYPD1, Ly6/PLAUR domain-containing protein 1, FLJ41033, LY6/PLAUR domain containing 1, LYPDC1, PHTS, Putative HeLa tumor suppressor
Source
Biotinylated Human LYPD1 Protein, His,Avitag (LY1-H82E6) is expressed from human 293 cells (HEK293). It contains AA Leu 21 - Gly 115 (Accession # Q8N2G4-1).
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 14.1 kDa. The protein migrates as 18-22 kDa and 35-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Biotinylated Human LYPD1 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-ELISA

Immobilized Biotinylated Human LYPD1 Protein, His,Avitag (Cat. No. LY1-H82E6) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Anti-LYPD1 antibody with a linear range of 0.008-0.125 μg/mL (QC tested).
Protocol
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