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Product Details
Synonyms
CD71, TFRC, TFR, sTfR, TfR1, Trfr, T9, p90
Source
Biotinylated Rat Transferrin R Protein, Avitag,His Tag (TFR-R82E3) is expressed from human 293 cells (HEK293). It contains AA Gly 87 - Phe 761 (Accession # Q99376-1).
Predicted N-terminus: Gly
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 79.8 kDa. The protein migrates as 70-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Rat Transferrin R Protein, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Rat Transferrin R Protein, Avitag,His Tag (Cat. No. TFR-R82E3) is more than 90% and the molecular weight of this protein is around 160-190 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Rat Transferrin R Protein, Avitag,His Tag (Cat. No. TFR-R82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse Transferrin Protein, Mouse IgG2a Fc Tag (Cat. No. TRN-M525b) with a linear range of 0.2-6 ng/mL (QC tested).
Protocol
Immobilized Mouse Transferrin Protein, Mouse IgG2a Fc Tag (Cat. No. TRN-M525b) at 2 μg/mL (100 μL/well) can bind Biotinylated Rat Transferrin R Protein, Avitag,His Tag (Cat. No. TFR-R82E3) with a linear range of 0.5-31 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Transferrin receptor protein 1
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
15 Details
- Highest Development Stage：
Approved
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