This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 56.7 kDa. The protein migrates as 60-68 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 20 mM MOPS, 150 mM NaCl, 5 mM CHAPS, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Rabbit Angiopoietin-2 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Rabbit Angiopoietin-2 Protein, His Tag (Cat. No. AN2-R53H5) at 2 μg/mL (100 μL/well) can bind Human TIE2, Fc Tag (Cat. No. TI2-H5255) with a linear range of 5-78 ng/mL (QC tested).
Immobilized Human TIE2, Fc Tag (Cat. No. TI2-H5255) at 5 μg/mL (100 μL/well) can bind Rabbit Angiopoietin-2 Protein, His Tag (Cat. No. AN2-R53H5) with a linear range of 2-39 ng/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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