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Your Position: Home > Protein > HLA-A*0201 | B2M | MAGE-A4 > HLM-H82E5

Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein (Monomer, MALS verified)

Streptavidin Protein - PE (STN-NP117, STN-NP119), supporting the assembly of your own MHC Tetramers!

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  • Source
    Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein(HLM-H82E5) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & GVYDGREHTV peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & GVYDGREHTV).
    Predicted N-terminus: Gly 25 & Ile 21
  • Molecular Characterization

    Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein is produced by co-expression of HLA and B2M loaded with MAGE-A4 peptide.

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein migrates as 42-43 kDa and 12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
HLA-A*0201 | B2M | MAGE-A4 SDS-PAGE

Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
HLA-A*0201 | B2M | MAGE-A4 MALS images

The purity of Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein (Cat. No. HLM-H82E5) is more than 95% and the molecular weight of this protein is around 48-60 kDa verified by SEC-MALS.

Bioactivity-ELISA
 HLA-A*0201 | B2M | MAGE-A4 ELISA

Immobilized Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein (Cat. No. HLM-H82E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).

 HLA-A*0201 | B2M | MAGE-A4 ELISA

Immobilized Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein (Cat. No. HLM-H82E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA-A2&MAGE-A4 Antibody, Human IgG1 with a linear range of 0.1-1 ng/mL (QC tested).

 HLA-A*0201 | B2M | MAGE-A4 ELISA

Immobilized Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein (Cat. No. HLM-H82E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can Anti-B2M Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (Routinely tested).

Bioactivity-SPR
 HLA-A*0201 | B2M | MAGE-A4 SPR

Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein (Cat. No. HLM-H82E5) captured on Biotin CAP-Series S Sensor Chip can bind Anti-HLA-A2&MAGE-A4 Antibody, Human IgG1 with an affinity constant of 22.3 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    The MAGE A4 antigen is a cancer-testis antigen and is expressed intracellularly in various solid tumor tissues, MAGE A4230-239 peptide (GVYDGREHTV) is a cytotoxic T lymphocyte (CTL) epitope presented by HLA-A2 The Human HLA-A*0201 MAGE-A4 (GVYDGREHTV) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and GVYDGREHTV peptide of the MAGE-A4.
  • Clinical and Translational Updates

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