This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 63.1 kDa. The protein migrates as 90-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Influenza A [Sydney/5/2021 (H1N1)] HA Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Influenza A [Sydney/5/2021 (H1N1)] HA Protein, His Tag (Cat. No. HA1-V52H4) is more than 90% and the molecular weight of this protein is around 215-245 kDa verified by SEC-MALS.
Immobilized Influenza A [Sydney/5/2021 (H1N1)] HA Protein, His Tag (Cat. No. HA1-V52H4) at 1 μg/mL (100 μL/well) can bind Human Anti-HA (H1N1) antibody3E1 with a linear range of 0.001-0.025 μg/mL (QC tested).
Human Anti-HA (H1N1) antibody3E1 captured on Protein A Chip can bind Influenza A [Sydney/5/2021 (H1N1)] HA Protein, His Tag (Cat. No. HA1-V52H4) with an affinity constant of 4.46 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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