Acrobiosystems for English
icon_bulk_orderBulk inquiry/Quick order
0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Protein > BCMA > BCA-HA2H7

Alexa Fluor™ 555-Labeled Human BCMA / TNFRSF17 Protein, His TagStar Staining

Order Now

  • Synonym
    TNFRSF17,CD269,BCM,BCMA
  • Source
    Alexa Fluor 555-Labeled Human BCMA, His Tag (BCA-HA2H7) is produced via conjugation of AF555 to Human BCMA, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human BCMA, His Tag is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 54 (Accession # Q02223-1).
    Predicted N-terminus: Met 1
  • Molecular Characterization
    BCMA Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 22.8 kDa.

  • Conjugate

    AF555

    Excitation Wavelength: 561 nm

    Emission Wavelength: 572 nm

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Evaluation of CAR expression
FACS Analysis of Anti-BCMA CAR Expression
 BCMA CAR_T

5e5 of anti-BCMA CAR-293 cells were stained with 100 μL of 3 μg/mL of Alexa Fluor 555-Labeled Human BCMA, His Tag (Cat. No. BCA- HA2H7) and negative control protein respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). Alexa 555 signal was used to evaluate the binding activity (QC tested).

FACS Analysis of Non-specific binding to PBMCs
 BCMA CAR_T

5e5 of PBMCs were stained with Alexa Fluor 555-Labeled Human BCMA, His Tag (Cat. No. BCA-HA2H7) and anti-CD3 antibody, washed and then analyzed with FACS. FITC signal was used to evaluate the expression of CD3+T cells in PBMCs, and Alexa 555 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).

  • Background
    Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) is also known as B-cell maturation protein (BCMA), CD antigen CD269, which is a member of the TNF-receptor superfamily. TNFRSF17 contains one TNFR-Cys repeat. TNFRSF17 is expressed in mature B-cells, but not in T-cells or monocytes. TNFRSF17 is receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. TNFRSF17 promotes B-cell survival and plays a role in the regulation of humoral immunity. TNFRSF17 can activate NF-kappa-B and JNK.
  • Clinical and Translational Updates

Comments (0)


ETA of in-stock products: 2 business days

Price(USD) : $420.00

Price(USD) : $2616.00

Promotion & Exhibitions



Drug Development Status

  • Number of Launched Drugs:7 Details
  • Number of Drugs in Clinical Trials:101 Details
  • Latest Research Phase:Approved

Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Related Products

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop
Call us
Call us
North America:
+1 800-810-0816 (Toll Free)
Asia & Pacific:
+86 400-682-2521
Fax
Fax
+1 888-377-6111
Address
Address
1 Innovation Way, Newark, DE 19711, USA

Leave a message