Product Details
Synonyms
FGL2, Fibroleukin, Pt49
Source
Human FGL2 (204-439) Protein, His Tag (FG2-H5243) is expressed from human 293 cells (HEK293). It contains AA Pro 204 - Pro 439 (Accession # Q14314-1).
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 29.6 kDa. The protein migrates as 36-41 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Human FGL2 (204-439) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS

The purity of Human FGL2 (204-439) Protein, His Tag (Cat. No. FG2-H5243) is more than 85% and the molecular weight of this protein is around 35-45 kDa verified by SEC-MALS.
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Bioactivity-ELISA

Immobilized Human FGL2 (204-439) Protein, His Tag (Cat. No. FG2-H5243) at 1 μg/mL (100 μL/well) can bind Anti-FGL2/Prothrombinase antibody with a linear range of 2-78 ng/mL (QC tested).
Protocol
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