Human FGL2 (204-439) Protein, His Tag (MALS verified)

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Cat. No. / Size
Price
Qty
FG2-H5243-100ug
$430.00
FG2-H5243-1mg (500ug X 2)
$2885.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    FGL2, Fibroleukin, Pt49

  • Source

    Human FGL2 (204-439) Protein, His Tag (FG2-H5243) is expressed from human 293 cells (HEK293). It contains AA Pro 204 - Pro 439 (Accession # Q14314-1).

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  • Molecular Characterization

    FGL2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 29.6 kDa. The protein migrates as 36-41 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    FGL2 SDS-PAGE

    Human FGL2 (204-439) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    FGL2 SEC-MALS

    The purity of Human FGL2 (204-439) Protein, His Tag (Cat. No. FG2-H5243) is more than 85% and the molecular weight of this protein is around 35-45 kDa verified by SEC-MALS.

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  • Bioactivity-ELISA

     FGL2 ELISA

    Immobilized Human FGL2 (204-439) Protein, His Tag (Cat. No. FG2-H5243) at 1 μg/mL (100 μL/well) can bind Anti-FGL2/Prothrombinase antibody with a linear range of 2-78 ng/mL (QC tested).

    Protocol

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Background

Fibrinogen-like protein 2, also know as FGL2 is a protein that exhibits pleiotropic effects within the body and is an important immune regulator of both innate and adaptive responses.[7] The protein exists as both a Type II transmembrane protein (with the carboxy terminus on the extracellular side of the plasma membrane) found on the surface of macrophages and endothelial cells and can be constitutively secreted by both CD4+ and CD8+ T cells.

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