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This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 101.6 kDa. The protein migrates as 95-120 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human Transferrin R Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Human Transferrin R Protein, Fc Tag (Cat. No. TFR-H5264) is more than 85% and the molecular weight of this protein is around 200-240 kDa verified by SEC-MALS.
Immobilized Human Transferrin Protein, His Tag (Cat. No. TRN-H52H3) at 2 μg/mL (100 μL/well) can bind Human Transferrin R Protein, Fc Tag (Cat. No. TFR-H5264) with a linear range of 0.039-0.625 μg/mL (QC tested).
Human Transferrin R Protein, Fc Tag (Cat. No. TFR-H5264) captured on Protein A Chip can bind Human Transferrin, His Tag (Cat. No. TRN-H4229) with an affinity constant of 0.491 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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