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This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 20.5 kDa. The protein migrates as 26-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human TNF-beta Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human TNF-beta Protein, His Tag (Cat. No. TNB-H52H3) is more than 90% and the molecular weight of this protein is around 62-78 kDa verified by SEC-MALS.
Immobilized Human TNFR1, Fc Tag (Cat. No. TN1-H5251) at 5 μg/mL (100 μL/well) can bind Human TNF-beta Protein, His Tag (Cat. No. TNB-H52H3) with a linear range of 1-20 ng/mL (QC tested).
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