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Your Position: Home > Protein > IFN-omega > IFA-H52H3

Human IFN-omega Protein, His Tag (BLI & MALS verified)

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  • Synonym
    IFNW1,IFN-omega
  • Source
    Human IFN-omega Protein, His Tag(IFA-H52H3) is expressed from human 293 cells (HEK293). It contains AA Cys 24-Ser 195 (Accession # P05000).
    Predicted N-terminus: Cys 24
  • Molecular Characterization
    IFN-omega Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 21.8 kDa. The protein migrates as 27-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IFN-omega SDS-PAGE

Human IFN-omega Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
IFN-omega MALS images

The purity of Human IFN-omega Protein, His Tag (Cat. No. IFA-H52H3) is more than 90% and the molecular weight of this protein is around 18-30 kDa verified by SEC-MALS.

Bioactivity-BLI
 IFN-omega BLI

Loaded Human IFN-omega Protein, His Tag (Cat. No. IFA-H52H3) on NTA Biosensor, can bind Human IFNAR1, Fc Tag (Cat. No. IF1-H5253) with an affinity constant of 245 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

  • Background
    The protein encoded by this gene is an interferon and possesses antiviral activity. The encoded protein binds to the interferon alpha/beta receptor but not to the interferon gamma receptor. This intronless gene has several pseudogenes spread throughout the genome.
  • Clinical and Translational Updates

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