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Your Position: Home > Protein > scFv control > DNP-H5254

scFv control Protein, Fc Tag (MALS verified)

  • Source
    Anti-DNP-scFv, Fc Tag (DNP-H5254) is expressed from human 293 cells (HEK293). It mimics the scFv presented on Anti-DNP.
  • Molecular Characterization

    This protein carries a human IgG1 Fc tag at the C-terminus

    The protein has a calculated MW of 52.8 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
scFv control SDS-PAGE

scFv control Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
scFv control MALS images

The purity of scFv control Protein, Fc Tag (Cat. No. DNP-H5254) is more than 85% and the molecular weight of this protein is around 125-145 kDa verified by SEC-MALS.

Bioactivity-ELISA
 scFv control ELISA

Immobilized DNP-Albumin Conjugate, Bovine (Cat. No. DNP-H5254) at 10 μg/mL (100 μL/well) can bind scFv control Protein, Fc Tag (Cat. No. DNP-H5254) with a linear range of 0.1-4 ng/mL (QC tested).

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DMF (Drug Master File)

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