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Your Position: Home > Protein > HLA-A*1101 | B2M | KRASG12D (VVGADGVGK) > HLD-H52H4

Human HLA-A*11:01&B2M&KRASG12D (VVGADGVGK) Complex Protein (Monomer, MALS verified)

Streptavidin Protein - PE (STN-NP117, STN-NP119), supporting the assembly of your own MHC Tetramers!

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  • Synonym
    HLA-A*1101 | B2M | KRASG12D (VVGADGVGK)
  • Source
    Human HLA-A*11:01&B2M&KRASG12D (VVGADGVGK) Complex Protein(HLD-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) & VVGADGVGK peptide (Accession # Q5S3G3-1 (HLA-A*11:01) & P61769 (B2M) & VVGADGVGK).
    Predicted N-terminus: Gly 25 & Ile 21
  • Molecular Characterization

    Human HLA-A*11:01&B2M&KRASG12D (VVGADGVGK) Complex Protein is produced by co-expression of HLA and B2M loaded with KRASG12D peptide.

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 36.0 kDa and 11.7 kDa. The protein migrates as 41-45 kDa and 14 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
HLA-A*1101 | B2M | KRASG12D (VVGADGVGK) SDS-PAGE

Human HLA-A*11:01&B2M&KRASG12D (VVGADGVGK) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
HLA-A*1101 | B2M | KRASG12D (VVGADGVGK) MALS images

The purity of Human HLA-A*11:01&B2M&KRASG12D (VVGADGVGK) Complex Protein (Cat. No. HLD-H52H4) is more than 90% and the molecular weight of this protein is around 45-60 kDa verified by SEC-MALS.

Bioactivity-ELISA
 HLA-A*1101 | B2M | KRASG12D (VVGADGVGK) ELISA

Immobilized Human HLA-A*11:01&B2M&KRASG12D (VVGADGVGK) Complex Protein (Cat. No. HLD-H52H4) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).

 HLA-A*1101 | B2M | KRASG12D (VVGADGVGK) ELISA

Immobilized Human HLA-A*11:01&B2M&KRASG12D (VVGADGVGK) Complex Protein (Cat. No. HLD-H52H4) at 1 μg/mL (100 μL/well) can bind Anti-B2M Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (Routinely tested).

Bioactivity-SPR
 HLA-A*1101 | B2M | KRASG12D (VVGADGVGK) SPR

Anti-HLA class I Antibody, Human IgG1 (W6/32) captured on Protein A Chip can bind Human HLA-A*11:01&B2M&KRASG12D (VVGADGVGK) Complex Protein (Cat. No. HLD-H52H4) with an affinity constant of 1.06 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    The Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) oncogene plays a critical role in the initiation and maintenance of pancreatic tumors and its signaling network represents a major target for therapeutic intervention. The Biotinylated Human HLA-A*1101 KRASG12D (VVGADGVGK) complex protein is a complex of HLA-A*1101 of the MHC Class I, B2M, and VVGADGVGK peptide of the KRASG12D.
  • Clinical and Translational Updates

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