Human HLA-A*11:01&B2M&KRASG12V (VVGAVGVGK) Tetramer Protein is assembled by biotinylated monomer and streptavidin.
Biotinylated Human HLA-A*11:01&B2M&KRASG12V (VVGAVGVGK) Complex Protein is produced by co-expression of HLA and B2M loaded with KRASG12V peptide. Biotinylated Human HLA-A*11:01&B2M&KRASG12V (VVGAVGVGK) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 36.0 kDa and 11.7 kDa. The protein migrates as 39-43 kDa, 14 kDa and 12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human HLA-A*11:01&B2M&KRASG12V (VVGAVGVGK) Tetramer Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Immobilized Human HLA-A*11:01&B2M&KRASG12V (VVGAVGVGK) Tetramer Protein (Cat. No. HLV-H52H8) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-4 ng/mL (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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