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Your Position: Home > Protein > HLA-A*1101 | B2M | KRAS (VVGAGGVGK) > HLS-HA2H9

Alexa Fluor™ 647-Labeled Human HLA-A*11:01&B2M&KRAS (VVGAGGVGK) Complex ProteinStar Staining (Monomer)

Streptavidin Protein - PE (STN-NP117, STN-NP119), supporting the assembly of your own MHC Tetramers!

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  • Synonym
    HLA-A*1101 | B2M | KRAS (VVGAGGVGK)
  • Source
    Alexa Fluor 647-Labeled Human HLA-A*11:01&B2M&KRAS (VVGAGGVGK) Complex Protein (HLS-HA2H9) is produced via conjugation of AF647 to Human HLA-A*11:01&B2M&KRAS (VVGAGGVGK) Complex Protein with a new generation site-specific technology under Star Staining labeling platform. Human HLA-A*11:01&B2M&KRAS (VVGAGGVGK) Complex Protein is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) & VVGAGGVGK peptide (Accession # Q5S3G3-1 (HLA-A*11:01) & P61769 (B2M) & VVGAGGVGK).
    Predicted N-terminus: Gly 25 & Ile 21
  • Molecular Characterization

    Alexa Fluor 647-Labeled Human HLA-A*11:01&B2M&KRAS (VVGAGGVGK) Complex Protein is produced by co-expression of HLA and B2M loaded with KRAS peptide.

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 36.4 kDa and 14.0 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    AF647

    Excitation Wavelength: 640 nm

    Emission Wavelength: 672 nm

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
HLA-A*1101 | B2M | KRAS (VVGAGGVGK) SDS-PAGE

Alexa Fluor 647-Labeled Human HLA-A*11:01&B2M&KRAS (VVGAGGVGK) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Background
    The Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) oncogene plays a critical role in the initiation and maintenance of pancreatic tumors and its signaling network represents a major target for therapeutic intervention. The Biotinylated Human HLA-A*1101 KRAS (VVGAGGVGK) complex protein is a complex of HLA-A*1101 of the MHC Class I, B2M, and VVGAGGVGK peptide of the KRAS.
  • Clinical and Translational Updates

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