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Your Position: Home > Protein > Glypican 3 > GP3-HA2H5

Alexa Fluor™ 488-Labeled Human Glypican 3 / GPC3 Protein, His TagStar Staining

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  • Synonym
    GPC3,OCI5,Glypican-3,GTR2-2,MXR7,DGSX,SDYS ,SGB,SGBS,SGBS1
  • Source
    Alexa Fluor 488-Labeled Human Glypican 3 Protein, His Tag (GP3-HA2H5) is produced via conjugation of AF488 to Human Glypican 3 Protein, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human Glypican 3 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # P51654-1 ).
    Predicted N-terminus: Gln 25
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 77.6 kDa.

  • Conjugate

    AF488

    Excitation Wavelength: 488 nm

    Emission Wavelength: 517 nm

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Evaluation of CAR expression
FACS Analysis of Anti-Glypican 3 CAR Expression
 Glypican 3 CAR_T

5e5 of anti-GPC3 CAR-293 cells were stained with 100 μL of 3 μg/mL of Alexa Fluor 488-Labeled Human Glypican 3 Protein, His Tag (Cat. No. GP3-HA2H5) and negative control protein respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). Alexa Fluor 488 signal was used to evaluate the binding activity (QC tested).

FACS Analysis of Non-specific binding to PBMCs
 Glypican 3 CAR_T

5e5 of PBMCs were stained with Alexa Fluor 488-Labeled Human Glypican 3 Protein, His Tag (Cat. No. GP3-HA2H5) and anti-CD3 antibody, washed and then analyzed with FACS. PE signal was used to evaluate the expression of CD3+ T cells in PBMCs, and Alexa Fluor 488 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).

  • Background
    Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.
  • Clinical and Translational Updates

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Price(USD) : $420.00

Price(USD) : $2616.00

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  • Number of Drugs in Clinical Trials:31 Details
  • Latest Research Phase:Phase 2 Clinical

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