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Your Position: Home > Protein > FAS Ligand > FAL-H5246

Cynomolgus / Rhesus macaque Fas Ligand / TNFSF6 Protein, His Tag, premium grade

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  • Source
    Cynomolgus / Rhesus macaque Fas Ligand Protein, His Tag, premium grade(FAL-H5246) is expressed from human 293 cells (HEK293). It contains AA Pro 133 - Leu 280 (Accession # P63308-1 ).
    Predicted N-terminus: His
    It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
  • Molecular Characterization
    FAS Ligand Structure

    This protein carries a polyhistidine tag at the N-terminus

    The protein has a calculated MW of 18.8 kDa. The protein migrates as 27-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Sterility
    The sterility testing was performed by membrane filtration method.
  • Mycoplasma
    Negative.
  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FAS Ligand SDS-PAGE

Cynomolgus / Rhesus macaque Fas Ligand Protein, His Tag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
FAS Ligand MALS images

The purity of Cynomolgus / Rhesus macaque Fas Ligand Protein, His Tag, premium grade (Cat. No. FAL-H5246) is more than 95% and the molecular weight of this protein is around 65-80 kDa verified by SEC-MALS.

Bioactivity-ELISA
 FAS Ligand ELISA

Immobilized Human Fas Protein, Fc Tag (Cat. No. FAS-H5252) at 5 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque Fas Ligand Protein, His Tag, premium grade (Cat. No. FAL-H5246) with a linear range of 1-31 ng/mL (QC tested).

 FAS Ligand ELISA

Immobilized Cynomolgus / Rhesus macaque Fas Ligand Protein, His Tag, premium grade (Cat. No. FAL-H5246) at 2 μg/mL (100 μL/well) can bind Human Fas Protein, Fc Tag (Cat. No. FAS-H5252) with a linear range of 0.1-1 ng/mL (Routinely tested).

  • Background
    Fas ligand is also known as FasL, CD178, CD95L, or TNFSF6, is a homotrimeric type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Its binding with its receptor induces apoptosis. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Mature human Fas Ligand consists of a 179 amino acid (aa) extracellular domain (ECD), a 22 aa transmembrane segment, and a 80 aa cytoplasmic domain. Within the ECD, human Fas Ligand shares 81% and 78% aa sequence identity with mouse and rat Fas Ligand, respectively. Apoptosis triggered by Fas-Fas ligand binding plays a fundamental role in the regulation of the immune system. Its functions include:T-cell homeostasis, cytotoxic T-cell activity, immune privilege, maternal tolerance, tumor counterattack. Defective Fas-mediated apoptosis may lead to oncogenesis as well as drug resistance in existing tumors. Germline mutation of Fas is associated with autoimmune lymphoproliferative syndrome (ALPS), a childhood disorder of apoptosis.
  • Clinical and Translational Updates

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ETD of in-stock products: 2 business days

Price(USD) : $410.00

Price(USD) : $3410.00

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