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Your Position: Home > Antibody > His > HIS-PLM535

HRP conjugated Anti-His Antibody (AY63), mAb

  • Source
    HRP conjugated Anti-His Antibody (AY63), mAb is a chimeric monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain. The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with His.
  • Isotype
    Human IgG1 | Human Kappa
  • Specificity
    This product is a specific antibody specifically reacts with His tag protein, and can recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
  • Application
    WB 1:1000-1:10000 (0.05-0.5 μg/mL); Elisa 1:3000-1:10000 (0.05-0.2 μg/mL).
  • Endotoxin
    Less than 1.0 EU per mg by the LAL method.
  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS,pH 7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70 °C for 6 months after reconstitution;
    3. 2-8 °C for 2-3 weeks under sterile conditions after reconstitution.

Immobilized Canine TNF-alpha, His Tag (Cat. No. TNA-C52H3) at 1 μg/mL (100 μL/well) can bind HRP conjugated Anti-His Antibody (AY63), mAb (Cat. No. HIS-PLM535) with a linear range of 0.2-13 ng/mL (QC tested).

  • Background
    His tag is a very small molecular weight tag, usually composed of 6-10 histidine (His). It is one of the commonly used tags for protein purification and detection. Due to its small molecular weight, fusion into the target protein has almost no effect on the structure and characteristics of the protein. Anti-his tag antibody can accurately detect, locate and purify His tag fusion protein, so as to provide convenience for the vast number of researchers.
  • Clinical and Translational Updates
  • Please contact us via if you have any question on this product.

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ETD of in-stock products: 2 business days

Price(USD) : $250.00

Price(USD) : $1680.00

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