Product Details
Source
HRP conjugated Anti-His Antibody (AY63), mAb is a recombinant chimeric monoclonal antibody expressed in HEK293, which combines the variable region of a mouse monoclonal antibody with human constant domain.
Antibody Type
Recombinant Monoclonal
Clone
AY63
Isotype
Human IgG1, Kappa
Reactivity
Tag
Immunogen
A protein peptide with his tag
Conjugate
HRP Labeled
Specificity
This product is a specific antibody specifically reacts with His tag protein, and can recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
Application
ApplicationRecommended UsageWestern Blot0.2-2 ug/mLELISA1:400-1:4000Purification
Protein A purified / Protein G purified.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70 °C for 6 months after reconstitution.
- 2-8 °C for 2-3 weeks under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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Performance Data
Bioactivity-ELISA

Immobilized Canine TNF-alpha, His Tag (Cat. No. TNA-C52H3) at 1 μg/mL (100 μL/well) can bind HRP conjugated Anti-His Antibody (AY63), mAb (Cat. No. HIS-PLM535) with a linear range of 0.2-13 ng/mL (QC tested).
Protocol
Western Blot

Detection of HRP conjugated Anti-His Tag Antibody (AY63), mAb by Western Blot. HRP conjugated Anti-His Antibody (AY63), mAb at 1/10000 dilution + Human LAG-3 / CD223 Protein, His Tag at 75-250ng. <br>Predicted band size: 60 kDa 12% Bis-Tris Protein Gel.
Customer Reviews Writing Reviews
E***t2026-04-21Likes
We ordered and tested the anti-His antibody from ACROBiosystems for routine Western blot and ELISA experiments, and we were very satisfied with both the product quality and the delivery time.
R***n2026-02-21Likes
I have ordered, ACROBiosystemsHRP conjugated Anti-His Antibody (AY63), mAb, for my ELISA experiment, working very good! May I have a PINK color llama? Thanks, -Raven
Background
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