HRP conjugated Anti-His Antibody (AY63), mAb

Customer Reviews
Cat. No. / Size
Price
Qty
HIS-PLM535-100ug
$265.00
HIS-PLM535-1mg (250ug X 4)
$1790.00
ETA of in-stock products:2 business days

Product Details

  • Source

    HRP conjugated Anti-His Antibody (AY63), mAb is a recombinant chimeric monoclonal antibody expressed in HEK293, which combines the variable region of a mouse monoclonal antibody with human constant domain.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    AY63

  • Isotype

    Human IgG1, Kappa

  • Reactivity

    Tag

  • Immunogen

    A protein peptide with his tag

  • Conjugate

    HRP Labeled

  • Specificity

    This product is a specific antibody specifically reacts with His tag protein, and can recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.

  • Application

    Application
    Recommended Usage
    Western Blot
    0.2-2 ug/mL
    ELISA
    1:400-1:4000
  • Purification

    Protein A purified / Protein G purified.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70 °C for 6 months after reconstitution.
    3. 2-8 °C for 2-3 weeks under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     His ELISA

    Immobilized Canine TNF-alpha, His Tag (Cat. No. TNA-C52H3) at 1 μg/mL (100 μL/well) can bind HRP conjugated Anti-His Antibody (AY63), mAb (Cat. No. HIS-PLM535) with a linear range of 0.2-13 ng/mL (QC tested).

    Protocol
  • Western Blot

     His WESTERN BLOT
  • Detection of HRP conjugated Anti-His Tag Antibody (AY63), mAb by Western Blot. HRP conjugated Anti-His Antibody (AY63), mAb at 1/10000 dilution + Human LAG-3 / CD223 Protein, His Tag at 75-250ng. <br>Predicted band size: 60 kDa 12% Bis-Tris Protein Gel.

Customer Reviews
Writing Reviews

  • E***t2026-04-21
    Likes
    We ordered and tested the anti-His antibody from ACROBiosystems for routine Western blot and ELISA experiments, and we were very satisfied with both the product quality and the delivery time.
  • R***n2026-02-21
    Likes
    I have ordered, ACROBiosystemsHRP conjugated Anti-His Antibody (AY63), mAb, for my ELISA experiment, working very good! May I have a PINK color llama? Thanks, -Raven

Background

His tag is a very small molecular weight tag, usually composed of 6-10 histidine (His). It is one of the commonly used tags for protein purification and detection. Due to its small molecular weight, fusion into the target protein has almost no effect on the structure and characteristics of the protein. Anti-his tag antibody can accurately detect, locate and purify His tag fusion protein, so as to provide convenience for the vast number of researchers.

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