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This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 27.8 kDa. The protein migrates as 30-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 51 mM Tris,100 mM Glycine,25 mM Arginine,150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human IgG1 Fc (C103S, N180A) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human IgG1 Fc (C103S, N180A) Protein, His Tag (Cat. No. IG1-H52H7) is more than 85% and the molecular weight of this protein is around 50-62 kDa verified by SEC-MALS.
Immobilized Human IgG1 Fc (C103S, N180A) Protein, His Tag (Cat. No. IG1-H52H7) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) with a linear range of 0.001-0.156 μg/mL (QC tested).
Immobilized Human IgG1 Fc (C103S, N180A) Protein, His Tag (Cat. No. IG1-H52H7) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD16a (V176), Avitag,His Tag (SPR & BLI verified) (Cat. No. CDA-H82E9) with a linear range of 0.039-1.25 μg/mL (Routinely tested).
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