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Biotinylated Human LY6G6D Protein, His,Avitag™ (HPLC verified)

Cat. No. / Size
Price
Qty
LYD-H82Q3-25ug
$360.00
LYD-H82Q3-200ug
$1420.00
ETA of in-stock products:2 business days
Sub-Total$ 0

Product Details

  • Synonyms

    lymphocyte antigen 6 family member G6D, C6orf23, G6D, LY6-D, MEGT1, NG25

  • Source

    Biotinylated Human LY6G6D Protein, His,Avitag (LYD-H82Q3) is expressed from human 293 cells (HEK293). It contains AA Asn 20 - Ser 104 (Accession # O95868-1).

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  • Molecular Characterization

    LY6G6D Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 12.8 kDa. The protein migrates as 15-18 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

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  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    LY6G6D SDS-PAGE

    Biotinylated Human LY6G6D Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-HPLC

    LY6G6D SEC-HPLC

    The purity of Biotinylated Human LY6G6D Protein, His,Avitag (Cat. No. LYD-H82Q3) was greater than 95% as determined by SEC-HPLC.

  • Bioactivity-ELISA

     LY6G6D ELISA

    Immobilized Biotinylated Human LY6G6D Protein, His,Avitag (Cat. No. LYD-H82Q3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse Anti-LY6G6D Antibody (3D6E10), IgG1 with a linear range of 0.004-0.125 μg/mL (QC tested).

    Protocol

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Background

LY6G6D belongs to a cluster of leukocyte antigen-6 (LY6) genes located in the major histocompatibility complex (MHC) class III region on chromosome 6. Members of the LY6 superfamily typically contain 70 to 80 amino acids, including 8 to 10 cysteines. Most LY6 proteins are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor that is directly involved in signal transduction.

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