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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a human IgG1 Fc tag
The protein has a calculated MW of 75.7 kDa. The protein migrates as ＞145 kDa under reducing (R) condition, and 135-235 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, PH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human OX40 Ligand Protein, Avitag,Fc Tag, premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Biotinylated Human OX40 Ligand Protein, Avitag,Fc Tag, premium grade (Cat. No. OXL-H82F5) is more than 90% and the molecular weight of this protein is around 175-205 kDa verified by SEC-MALS.
Immobilized Human OX40 Protein, His Tag (Cat. No. OX0-H5224) at 1 μg/mL (100 μL/well) can bind Biotinylated Human OX40 Ligand Protein, Avitag,Fc Tag, premium grade (Cat. No. OXL-H82F5) with a linear range of 0.2-8 ng/mL (QC tested).
Immobilized Human Olfactory Marker Protein, His Tag (Cat. No. OMP-H5145) at 1 μg/mL (100 μL/well) can bind Biotinylated Human OX40 Ligand Protein, Avitag,Fc Tag, premium grade (Cat. No. OXL-H82F5) with a linear range of 0.1-8 ng/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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