This protein carries no "tag"
The protein has a calculated MW of 25.1 kDa. The protein migrates as 35-45 kDa under reducing (R) condition, and 50-58 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human M-CSF Protein, premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human M-CSF Protein, premium grade (Cat. No. MCF-H5218) is more than 95% and the molecular weight of this protein is around 65-75 kDa verified by SEC-MALS.
Immobilized Human M-CSF Protein, premium grade (Cat. No. MCF-H5218) at 5 μg/mL (100 μL/well) can bind Human M-CSF R, His Tag (Cat. No. CSR-H5228) with a linear range of 0.01-0.156 μg/mL (QC tested).
Immobilized Human M-CSF Protein, premium grade (Cat. No. MCF-H5218) at 5 μg/mL (100 μL/well) can bind Biotinylated Human M-CSF R, Avitag,His Tag (Cat. No. CSR-H82E0) with a linear range of 2-78 ng/mL (Routinely tested).
Immobilized Human M-CSF Protein, premium grade (Cat. No. MCF-H5218) at 5 μg/mL (100 μL/well) can bind Human M-CSF R Protein, Fc Tag (Cat. No. CSR-H5258) with a linear range of 0.2-2 ng/mL (Routinely tested).
Immobilized Human M-CSF Protein, premium grade (Cat. No. MCF-H5218) at 5 μg/mL (100 μL/well) can bind Human M-CSF R Protein, Mouse IgG2a Fc Tag (Cat. No. CSR-H5255) with a linear range of 0.2-2 ng/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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