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Your Position: Home > Protein > M-CSF > MCF-H5218

Human M-CSF / CSF-1 Protein, premium grade

  • Synonym
    M-CSF,CSF-1,Lanimostim
  • Source
    Human M-CSF Protein, premium grade(MCF-H5218) is expressed from human 293 cells (HEK293). It contains AA Glu 33 - Arg 255 (Accession # P09603-1 ).
    Predicted N-terminus: Glu 33
    It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
  • Molecular Characterization
    M-CSF Structure

    This protein carries no "tag"

    The protein has a calculated MW of 25.1 kDa. The protein migrates as 35-45 kDa under reducing (R) condition, and 50-58 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Sterility
    The sterility testing was performed by membrane filtration method.
  • Mycoplasma
    Negative.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
M-CSF SDS-PAGE

Human M-CSF Protein, premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
M-CSF MALS images

The purity of Human M-CSF Protein, premium grade (Cat. No. MCF-H5218) is more than 95% and the molecular weight of this protein is around 65-75 kDa verified by SEC-MALS.

Bioactivity-ELISA
 M-CSF ELISA

Immobilized Human M-CSF Protein, premium grade (Cat. No. MCF-H5218) at 5 μg/mL (100 μL/well) can bind Human M-CSF R, His Tag (Cat. No. CSR-H5228) with a linear range of 0.01-0.156 μg/mL (QC tested).

 M-CSF ELISA

Immobilized Human M-CSF Protein, premium grade (Cat. No. MCF-H5218) at 5 μg/mL (100 μL/well) can bind Biotinylated Human M-CSF R, Avitag,His Tag (Cat. No. CSR-H82E0) with a linear range of 2-78 ng/mL (Routinely tested).

 M-CSF ELISA

Immobilized Human M-CSF Protein, premium grade (Cat. No. MCF-H5218) at 5 μg/mL (100 μL/well) can bind Human M-CSF R Protein, Fc Tag (Cat. No. CSR-H5258) with a linear range of 0.2-2 ng/mL (Routinely tested).

 M-CSF ELISA

Immobilized Human M-CSF Protein, premium grade (Cat. No. MCF-H5218) at 5 μg/mL (100 μL/well) can bind Human M-CSF R Protein, Mouse IgG2a Fc Tag (Cat. No. CSR-H5255) with a linear range of 0.2-2 ng/mL (Routinely tested).

  • Background
    The colony stimulating factor 1 (CSF1), also known as macrophage colony-stimulating factor (M-CSF), is a secreted cytokine which influences hematopoietic stem cells to differentiate into macrophages or other related cell types. Eukaryotic cells also produce M-CSF in order to combat intercellular viral infection. It is one of the three experimentally described colony-stimulating factors. M-CSF binds to the colony stimulating factor 1 receptor. Macrophage colony-stimulating factor has been shown to interact with PIK3R2. M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. Locally produced M-CSF in the vessel wall contributes to the development and progression of atherosclerosis.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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