Save Product
Product Details
Synonyms
Spike, S protein, Spike glycoprotein, S glycoprotein
Source
MERS Spike protein trimer (R748A, R751A, V1060P, L1061P), His Tag (SPN-M52H5) is expressed from human 293 cells (HEK293). It contains AA Tyr 18 - Trp 1295 (Accession # K0BRG7 (R748A, R751A, V1060P, L1061P)). The recombinant protein has a T4 fibritin trimerization motif. Proline substitutions (V1060P, L1061P) and alanine substitutions (R748A, R751A) are introduced to stabilize the trimeric prefusion state of MERS-CoV Spike protein and abolish the furin cleavage site, respectively.
Predicted N-terminus: Tyr 18
Request for sequenceMolecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 145.9 kDa. The protein migrates as 150 kDa and 175-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Customers Also Viewed
Performance Data
SDS-PAGE
MERS Spike protein trimer (R748A, R751A, V1060P, L1061P), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of MERS Spike protein trimer (R748A, R751A, V1060P, L1061P), His Tag (Cat. No. SPN-M52H5) is more than 90% and the molecular weight of this protein is around 540-580 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human DPPIV, Fc Tag (Cat. No. DP4-H5266) at 5 μg/mL (100 μL/well) can bind MERS Spike protein trimer, His Tag (Cat. No. SPN-M52H5) with a linear range of 0.1-3 ng/mL (QC tested).
Protocol
Immobilized MERS Spike protein trimer, His Tag (Cat. No. SPN-M52H5) at 5 μg/mL (100 μL/well) can bind Human DPPIV, Fc Tag (Cat. No. DP4-H5266) with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
Customer Reviews Writing Reviews
Background
Recent Advances
Drug Development Progress- English Name：
Coronavirus spike glycoprotein
- Category：
- Approved Drugs：
15 Details
- Drugs in Clinical Trials：
88 Details
- Highest Development Stage：
Approved
Contact Us
[North America]: +1 800-810-0816
[Switzerland]: +41 800 040 012
[Asia & Pacific]: +86 400-682-2521
Drug Candidate Licensing
