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This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 145.9 kDa. The protein migrates as 150 kDa and 175-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
MERS Spike protein trimer (R748A, R751A, V1060P, L1061P), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of MERS Spike protein trimer (R748A, R751A, V1060P, L1061P), His Tag (Cat. No. SPN-M52H5) is more than 90% and the molecular weight of this protein is around 540-580 kDa verified by SEC-MALS.
Immobilized Human DPPIV, Fc Tag (Cat. No. DP4-H5266) at 5 μg/mL (100 μL/well) can bind MERS Spike protein trimer, His Tag (Cat. No. SPN-M52H5) with a linear range of 0.1-3 ng/mL (QC tested).
Immobilized MERS Spike protein trimer, His Tag (Cat. No. SPN-M52H5) at 5 μg/mL (100 μL/well) can bind Human DPPIV, Fc Tag (Cat. No. DP4-H5266) with a linear range of 0.1-2 ng/mL (Routinely tested).
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