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Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His Tag (MALS & SPR verified)

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  • Synonym
    HN protein
  • Source
    Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His Tag(HNN-M52H3) is expressed from human 293 cells (HEK293). It contains AA Gly 106 - Thr 582 (Accession # P11235).
    Predicted N-terminus: Gly 106
  • Molecular Characterization
    HN protein Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 54.6 kDa. The protein migrates as 66-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
HN protein SDS-PAGE

Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

HN protein MALS images

The purity of Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His Tag (Cat. No. HNN-M52H3) is more than 90% and the molecular weight of this protein is around 63-78 kDa verified by SEC-MALS.

 HN protein SPR

Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His Tag (Cat. No. HNN-M52H3) immobilized on CM5 Chip can bind 3'-Sialyllactose / GM3 Ganglioside oligosaccharide with an affinity constant of 586 μM as determined in a SPR assay (Biacore 8K) (QC tested).

  • Background
    Mumps immunity is typically assessed by measuring neutralizing-antibody responses directed against mumps HN and F proteins.
  • Clinical and Translational Updates

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