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Product Details
Synonyms
AP-TNAP, APTNAP, HOPS, HPPA, HPPC, HPPI, HPPO, TNALP, TNAP, TNS-ALP, TNSALP
Source
Human ALPL Protein, His Tag (ALL-H52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 18 - Ser 502 (Accession # P05186-1).
Predicted N-terminus: Leu 18
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 55.3 kDa. The protein migrates as 65-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Supplied as 0.2 μm filtered solution in 20 mM Tris, 150 mM NaCl, Glycerol, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human ALPL Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human ALPL Protein, His Tag (Cat. No. ALL-H52H3) is more than 90% and the molecular weight of this protein is around 115-135 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human ALPL Protein, His Tag (Cat. No. ALL-H52H3) at 1 μg/mL (100 μL/well) can bind various dilution ratio of Anti-ALPL Polyclonal Antibody (QC tested).
Protocol
Bioactivity
Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is >80,000 pmol/min/μg (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Alkaline phosphatase, tissue-nonspecific isozyme
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
3 Details
- Highest Development Stage：
Approved
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