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Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Complex Protein (Monomer, MALS verified)

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HLM-H82E1-25ug
$125.00
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HLM-H82E1-200ug
$700.00
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HLM-H82E1
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Product Details
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  • Product Details
  • Performance Data
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Synonyms

HLA-A*0201 & B2M & MAGE-A1 (KVLEYVIKV)

Source

Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Complex Protein (HLM-H82E1) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & KVLEYVIKV peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & KVLEYVIKV).

Predicted N-terminus: Gly 25 & Ile 21

Molecular Characterization

Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Complex Protein is produced by co-expression of HLA and B2M loaded with MAGE-A1 peptide.

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein migrates as 38-44 kDa and 12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Purity

>90% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System

Background

The MAGE A1 antigen is a cancer-testis antigen and is expressed intracellularly in various solid tumor tissues, MAGE A1 278-286 peptide (KVLEYVIKV) is a cytotoxic T lymphocyte (CTL) epitope presented by HLA-A2 The Human HLA-A*0201 MAGE-A1 (KVLEYVIKV) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and KVLEYVIKV peptide of the MAGE-A1.