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This protein carries a polyhistidine tag at the N-terminus and a twin strep tag at the C-terminus.
The protein has a calculated MW of 27.5 kDa. The protein migrates as 25-27 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
>90% as determined by SDS-PAGE.
This product is not suitable for cell based experiments due to cytotoxicity of DDM.
DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove DDM and CHS.
DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.
Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human Claudin-4 Protein, His,Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Immobilized Human Claudin-4 Protein, His,Twin-Strep Tag (Cat. No. CL4-H5587) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human Claudin 4 antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).
Human Anti-CLDN4 antibody-xi5A5, Human IgG1 captured on Protein A Chip can bind Human Claudin-4 Protein, His,Twin-Strep Tag (Cat. No. CL4-H5587) with an affinity constant of 2.77 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Price(USD) : $805.00
Price(USD) : $2985.00
Price(USD) : $7480.00
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